Table 1

Results of canonical phylogenetic ordination of AFLP data


Variation explained¶

Node ¥
 Marginal effects† λ1
 Conditional effects‡ λa
 P-Value#
Phylogenetic groups according to mtDNA-based phylogeny



S. galilaeus sensu lato incl. Barombi taxa *
 1
 0.15
 0.10
 0.000
S. galilaeus sensu lato w/o S. g. sanagaensis incl. Barombi taxa
 2
 0.12
 0.05
 n.s.
S. galilaeus w/o S. g. sanagaensis excluding Barombi taxa
 3
 0.15
 -
 n.s.
S. g. multifasciatus *
 4
 0.18
 0.13
 0.000
S. galilaeus w/o S. g. sanagaensis and S. g. multifasciatus
 5
 0.12
 -
 n.s.
S. galilaeus "Meme" *
 6
 0.10
 0.08
 0.000
"Cross-clade" + S. g. "Niger"
 7
 0.10
 -
 n.s.
"Cross-clade" *
 8
 0.09
 0.07
 0.001
"Barombi Mbo clade" *
 9
 0.18
 0.11
 0.000
Stomatepia ssp.*
 10
 0.08
 0.06
 0.000
St. mongo *
 11
 0.07
 0.05
 0.054
St. mariae *
 12
 0.06
 0.05
 0.008
St. pindu *
 13
 0.05
 -
 n.s.
St. mariae – St. pindu
 14
 0.06
 -
 n.s.
Pungu maclareni – Konia ssp.
 15
 0.07
 0.05
 0.025
Konia ssp. *
 16
 0.06
 0.06
 0.001
Pungu maclareni *
 17
 0.06
 -
 n.s.
Konia eisentrauti *
 18
 0.05
 0.04
 n.s.
Konia dikume *
 19
 0.05
 -
 n.s.
Myaka myaka *
 20
 0.05
 0.04
 n.s.
Sarotherodon lohbergeri *
 21
 0.06
 0.04
 n.s.
Sarotherodon steinbachi *
 22
 0.05
 -
 n.s.
"Barombi Sarotherodon clade"
 23
 0.08
 -
 n.s.
Phylogenetic groups according to AFLP-phylogeny



Myaka + S. caroli/S.linnellii
 27
 0.07
 -
 n.s.
Pungu + S. lohbergeri/S. steinbachi
 28
 0.07
 -
 n.s.
St. mongo + St. pindu
 29
 0.07
 -
 n.s.
S. galilaeus s. l. incl. Barombi taxa w/o S.g.multifasciatus and S.g. "Niger"
 30
 0.21
 0.21
 0.000
S. lohbergeri + S. steinbachi
 31
 0.06
 -
 n.s.
S. linnellii + S. caroli
 32
 0.06
 -
 n.s.
S. sp. "mudfeeder" + S. sp. "bighead"
 33
 0.08
 0.05
 0.013
Hypothetical ancient syngameons according to conflict between mtDNA-based and AFLP-based phylogenetic hypotheses
 groups ¥¥


P. maclareni + Konia ssp. + S. lohbergeri + S. steinbachi
 red (24)
 0.08
 0.06
 0.002
M. myaka + S. caroli + S. linnellii + S. lohbergeri + S. steinbachi
 green (25)
 0.09
 -
 n.s.
S. g. sanagaensis + S. galilaeus w/o S. g. multifasciatus. S. g. "Niger"
 blue (26)
 0.15
 -
 n.s.

Each phylogenetic group as identified by nodes in phylogenetic analyses of mtDNA-, or AFLP-data or by the conflict between the data sets (hypothetical syngameons) was tested for their explanatory value in explaining variance in the AFLP data set.

¥ Node numbers correspond to encircled node numbers for mtDNA-clades and AFLP-clades supported in Fig. 1.

¥¥ Groups refer to hypothetical syngameons as indicated by coloured blocks in Fig. 1a and b.

¶ "Variation explained" refers to eigenvalues of each phylogenetic group that explain part of the variation in the pruned AFLP-data (2355 loci / 33 samples identical to those in Fig. 1a. Sum of all unconstrained eigenvalues was 1.667, sum of all canonical eigenvalues 1.179.

† Marginal effects refer to eigenvalues ("fit") of each phylogenetic group if taken singly as the only variable on the pruned AFLP data set.

‡ Conditional effects refer to the increase in eigenvalues ("additional fit") of each phylogenetic group as selected by automatic forward selection.

# P-values refer to the significance level of the conditional effects as obtained with a Monte Carlo permutation test under the full model with 9999 random permutations.

* mtDNA-clades as indicated in Fig. 1b that are compatible with clades as identified by the AFLP phylogenetic tree in Fig. 1a.

Schliewen and Klee Frontiers in Zoology 2004 1:5   doi:10.1186/1742-9994-1-5