Comparison of volume renderings based on tomographic reconstructions of the posterior part of the fish head of a black molly (A-C) and S. tinanti (D-F), including the ears of the same specimens before (A, D) and after phosphotungstic acid staining (B-C, E-F). Without contrast enhancement, only bones and otoliths can be visualized (A, D). Phosphotungstic acid distinctly enhances tissue x-ray contrast enabling 3D reconstructions of the inner ears including the sensory epithelia (maculae and cristae) (C, F). Brain (orange): Ce, cerebellum; dM, dorsal medulla; Hy, hypophysis; H, hypothalamus; TO, tectum opticum; ears (transparent blue): c, cristae (green, yellow, blue); lot, lagenar otolith (yellow); sot, saccular otolith (purple); uot, utricular otolith (red). Scale bars, 1 mm.
Schulz-Mirbach et al. Frontiers in Zoology 2013 10:63 doi:10.1186/1742-9994-10-63