Enhancement of tissue contrast by performing PTA staining. In transverse tomographic sections of the unstained (A, D) or unstained and dehydrated samples (B, E) of both species (A-C, black molly; D-F, S. tinanti) only bone, pharyngeal teeth and otoliths can easily be identified while after staining (C, F) diverse other tissues and organs such as brain, muscles, and the membranous labyrinth are now clearly visible. All scans were performed using the same isotropic voxel size of 10 μm for both specimens and all three conditions. Scale bar, 1 mm.
Schulz-Mirbach et al. Frontiers in Zoology 2013 10:63 doi:10.1186/1742-9994-10-63