Table 1

Aliphatic cuticular substances found in Crematogaster modiglianii and the two varieties of Camponotus rufifemur

substance
 substance class
 retention index
 red Ca. rufifemur
 black Ca. rufifemur
 Cr. modiglianii
1
 C21
 n-alkane
 21
0.26 ± 0.01%
2
 C23:1
 n-alkene
 22.75
0.46 ± 0.03%
3
 unknown
 unknown
 22.9
0.20 ± 0.01%
4
 C23
 n-alkane
 23
0.16 ± 0.01%
5
 C24:1
 n-alkene
 23.78
0.49 ± 0.06%
6
 Docosenal+
 aldehyde
 24.07
0.40 ± 0.09%
7
 Docosenal+
 aldehyde
 24.12
0.20 ± 0.16%
8
 unknown
 unknown
 24.35
0.23 ± 0.06%
9
 12-MeC24
 branched alkane
 24.37
 0.06 ± 0.05%

10
 11-MeC24
 branched alkane
 24.39
0.45 ± 0.02%
11
 C25
 n-alkane
 25


12
 Tricosenal+
 aldehyde
 25.11
0.11 ± 0.09%
13
 unknown
 unknown
 25.69
 0.05 ± 0.02%

14
 unknown
 unknown
 25.7
0.67 ± 0.17%
15
 C26
 n-alkane
 26
 0.02 ± 0.01%

16
 Tetracosenal+
 aldehyde
 26.09
0.72 ± 0.17%
17
 unknown
 unknown
 26.37
0.21 ± 0.03%
18
 unknown
 unknown
 26.72
0.08 ± 0.02%
19
 C27
 n-alkane
 27
 0.13 ± 0.11%
 0.13 ± 0.01%
20
 Pentacosenal+
 aldehyde
 27.15
0.47 ± 0.39%
21
 unknown
 unknown
 27.71
 0.09 ± 0.04%

22
 unknown
 unknown
 27.73
0.52 ± 0.08%
23
 C28
 n-alkane
 28
 0.09 ± 0.06%
 0.14 ± 0.01%
24
 C29
 n-alkane
 29
 0.21 ± 0.17%
 0.27 ± 0.02%
25
 C30
 n-alkane
 30
 0.01 ± 0.01%
 0.20 ± 0.03%
26
 C31
 n-alkane
 31
 0.15 ± 0.11%

27
 C32
 n-alkane
 32
0.08 ± 0.03%
28
 C35:1
 n-alkene
 34.85
0.26 ± 0.09%
 0.15 ± 0.04%
29
 C35
 n-alkane
 35.05
0.15 ± 0.06%*
 0.76 ± 0.11%
30
 17-MeC35, 15-MeC35, 13-MeC35
 branched alkane
 35.31

0.88 ± 0.25%
31
 3-MeC35
 branched alkane
 35.74

0.3 ± 0.13%
32
 C37:2
 n-alkadiene
 36.42

2.31 ± 0.28%
33
 C37:2
 n-alkadiene
 36.51

1.56 ± 0.12%
34
 C37:2
 n-alkadiene
 36.64

0.45 ± 0.07%
35
 C37-13-ene, C37-14-ene, C37-15-ene, C37-16-ene
 n-alkene
 36.72

5.43 ± 0.49%
36
 C37-9-ene
 n-alkene
 36.86
0.48 ± 0.15%
 4.53 ± 0.4%
37
 25-MeC37-14-ene, 25-MeC37-16-ene++
 branched alkene
 36.96
 0.44 ± 0.07%

38
 C37
 n-alkane
 37.05

0.52 ± 0.1%
39
 19-MeC37, 17-MeC37, 15-MeC37, 13-MeC37, 11-MeC37
 branched alkane
 37.31

11.47 ± 0.28%
40
 C38:2
 n-alkadiene
 37.45

0.18 ± 0.09%
41
 11,27-DiMeC37, 11,25-DiMeC37
 branched alkane
 37.58

6.02 ± 0.33%
42
 unknown
 unknown
 37.79

0.63 ± 0.1%
43
 x(25,26,27)-MeC38-y(13,14,15,16)-ene++§
 branched alkene
 37.93
 1.99 ± 0.11%

44
 C39:3
 n-alkatriene
 38.23

1.12 ± 0.13%
45
 C39:3
 n-alkatriene
 38.3

1.46 ± 0.19%
46
 C39:2
 n-alkadiene
 38.43

15.23 ± 0.76%
47
 C39:2
 n-alkadiene
 38.53

13.7 ± 0.73%
48
 C39-ene
 n-alkene
 38.73

3.66 ± 0.34%
49
 unknown
 unknown
 38.79
 0.55 ± 0.08%

50
 C39:1
 n-alkene
 38.79

7.62 ± 0.19%
51
 C39:1
 n-alkene
 38.88

1.7 ± 0.08%
52
 27-MeC39-14-ene, 27-MeC39-16-ene
 branched alkene
 39.02
 88.66 ± 0.53%
3.15 ± 1.18%
53
 19-MeC39, 17-MeC39, 15-MeC39, 13-MeC39, 11-MeC39
 branched alkane
 39.29
 0.52 ± 0.24% (only 13-MeC39)
4.51 ± 0.2%
54
 11,21-DiMeC39, 11,23-DiMeC39, 11,27-DiMeC39, 11,29-DimeC39
 branched alkane
 39.54

4.84 ± 0.52%
55
 unknown
 unknown
 39.76
 0.22 ± 0.01%

56
 27-MeC40-14-ene, 27-MeC40-15-ene, 27-MeC40-16-ene++
 branched alkene
 39.97
 3.41 ± 0.09%

57
 unknown
 unknown
 40.17

1.04 ± 0.18%
58
 C40:3
 n-alkatriene
 40.35

0.36 ± 0.08%
59
 C40:2
 n-alkadiene
 40.42

3.39 ± 0.24%
60
 C40:2
 n-alkadiene
 40.57

3.01 ± 0.29%
61
 x(27,29)-MeC41-y(14,16,18)-ene++§
 branched alkene
 40.94
 3.35 ± 0.4%

62
 unknown
 unknown
 44.54
0.65 ± 0.12%
63
 unknown
 unknown
 44.68
0.45 ± 0.03%
64
 unknown
 unknown
 44.96
3.34 ± 0.19%
65
 C45:1
 n-alkene
 45.05
3.01 ± 0.04%
66
 36-MeC45:1
 branched alkene
 45.18
4.17 ± 0.06%
67
 unknown
 unknown
 45.49
1.09 ± 0.4%
68
 unknown
 unknown
 45.89
2.10 ± 0.16%
69
 unknown
 unknown
 45.97
0.98 ± 0.06%
70
 unknown
 unknown
 46.11
1.07 ± 0.06%
71
 C47:2
 n-alkadiene
 46.41
15.11 ± 0.52%
72
 C47:2
 n-alkadiene
 46.67
8.72 ± 0.37%
73
 C47:1
 n-alkene
 46.74
4.43 ± 0.18%
74
 C48:1
 n-alkene
 46.88
22.95 ± 0.96%
75
 C48:1
 n-alkene
 47.10
4.10 ± 0.07%
76
 38-MeC47:1
 branched alkene
 47.16
9.20 ± 0.49%
77
 unknown
 unknown
 47.42
1.49 ± 0.12%
78
 unknown
 unknown
 47.46
1.29 ± 0.11%
79
 unknown
 unknown
 47.81
1.91 ± 0.13%
80
 unknown
 unknown
 48.01
0.54 ± 0.07%
81
 C49:2
 n-alkadiene
 48.35
2.55 ± 1.7%
82
 C49:2
 n-alkadiene
 48.45
2.40 ± 1.6%
83
 C49:1
 n-alkene
 48.59
1.25 ± 0.11%

Relative peak areas (mean and standard error) for Ca. rufifemur and Cr. modiglianii are given based on FID data from n = 6 (red Ca. rufifemur), 3 (black Ca. rufifemur), and 8 (Cr. modiglianii) colonies. *found in less than 50% of the samples, +tentatively identified, ++position of double bond tentative, § number of substances and their exact structure could not be further determined. Retention indices beyond 44 are extrapolated.

Menzel et al. Frontiers in Zoology 2008 5:16   doi:10.1186/1742-9994-5-16