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Behavioral and physiological female responses to male sex ratio bias in a pond-breeding amphibian

Kristine L Grayson12*, Stephen P De Lisle1, Jerrah E Jackson3, Samuel J Black4 and Erica J Crespi5

Author Affiliations

1 Department of Biology, University of Virginia, Charlottesville, VA, 22904, USA

2 Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, USA

3 Mountain Lake Biological Station, University of Virginia, Pembroke, VA, 24136, USA

4 Department of Biology, Vassar College, Poughkeepsie, NY, 12604, USA

5 School of Biological Sciences, Washington State University, Pullman, WA, USA

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Frontiers in Zoology 2012, 9:24  doi:10.1186/1742-9994-9-24

Published: 18 September 2012

Abstract

Introduction

The phenomenon of sexual conflict has been well documented, and in populations with biased operational sex ratios the consequences for the rarer sex can be severe. Females are typically a limited resource and males often evolve aggressive mating behaviors, which can improve individual fitness for the male while negatively impacting female condition and fitness. In response, females can adjust their behavior to minimize exposure to aggressive mating tactics or minimize the costs of mating harassment. While male-male competition is common in amphibian mating systems, little is known about the consequences or responses of females. The red-spotted newt (Notophthalmus viridescens) is a common pond-breeding amphibian with a complex, well-studied mating system where males aggressively court females. Breeding populations across much of its range have male-biased sex ratios and we predicted that female newts would have behavioral mechanisms to mitigate mating pressure from males. We conducted four experiments examining the costs and behavioral responses of female N. viridescens exposed to a male-biased environment.

Results

In field enclosures, we found that female newts exposed to a male-biased environment during the five-month breeding season ended with lower body condition compared to those in a female-biased environment. Shorter-term exposure to a male-biased environment for five weeks caused a decrease in circulating total leukocyte and lymphocyte abundance in blood, which suggests females experienced physiological stress. In behavioral experiments, we found that females were more agitated in the presence of male chemical cues and females in a male-biased environment spent more time in refuge than those in a female-biased environment.

Conclusions

Our results indicate that male-biased conditions can incur costs to females of decreased condition and potentially increased risk of infection. However, we found that females can also alter their behavior and microhabitat use under a male-biased sex ratio. Consistent with surveys showing reduced detection probabilities for females, our research suggests that females avoid male encounters using edge and substrate habitat. Our work illustrates the integrated suite of impacts that sexual conflict can have on the structure and ecology of a population.

Keywords:
Leukocytes; Male-biased sex ratio; Mating behavior; Microhabitat use; Notophthalmus viridescens; Red-spotted newt; Sexual conflict; Stress