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Eel osmotic stress transcriptional factor 1 (Ostf1) is highly expressed in gill mitochondria-rich cells, where ERK phosphorylated

William KF Tse12*, Sheung C Chow1 and Chris KC Wong1*

Author Affiliations

1 Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong

2 Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA

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Frontiers in Zoology 2012, 9:3  doi:10.1186/1742-9994-9-3

Published: 10 March 2012

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Additional file 1:

Figure S1 Negative control of immunohistochemical staining experiments using the non-immune mouse or rabbit serum. No positive signals of Ostf1, pERK, and Cftr were found in the gill epithelia prepared from Japanese eels (Anguilla japonica) acclimated in seawater (day 7). Scale bar = 80 μm. Figure S2: The specificity of the Ostf1 antibody was confirmed by Western blotting. A band at 30 kDa was identified in the gill sample. Actin was used as the loading control.

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